Recombinant:Article Title: Protocol for generating a 3D hydrogel-based tumor resection model in vitro using pancreatic cancer cells.
Article Snippet: .. MATERIALS AND EQUIPMENT REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle Medium (DMEM) Pan Biotec Cat#F0445 Fetal bovine serum Pan Biotec Cat# P40-1401 Penicillin-streptomycin Pan Biotec Cat# P06-07100 L-glutamine Pan Biotec Cat# P04-80100 Phosphate buffered saline Pan Biotec Cat# P04-36500 Hydrogel R&D Systems Cat#3432-005-01 DAPI Carl Roth Cat#6335.1 Vybrant DiD Cell Labeling Solution Thermo Fisher Cat#V22887 SYTOX Green Thermo Fisher Cat#S7020 Experimental models: Cell lines Panc-1 ATCC Cat#CRL-1469 Software and algorithms Harmony 4.9 PerkinElmer N/A BioRender BioRender RRID: SCR_018361 Other Breeding incubator Binder Cat#9010-0319 96-pin punch plate Miebach et al. 1 N/A Motorized, computer-controlled xyz table CNC Cat#13110000 Biopsy punchout KAI Europe Cat#49301 Disposable syringe BD Cat#115256 Microplate reader Tecan Cat#30050303 High-content imaging system PerkinElmer Cat#HH16000000 Complete Dulbecco’s Modified Eagle Medium (DMEM) Reagent Final concentration Amount Dulbecco’s Modified Eagle Medium (DMEM) 88% 88 mL Fetal bovine serum 10% 10 mL Penicillin-streptomycin 100 U/mL - 100 μg/mL 1 mL L-glutamine 2 mM 1 mL Total N/A 100 mL The medium can be stored for 2–3 months at 4 ◦ C. STAR Protocols 7, 104416, March 20, 2026 5 ..
Modification:Article Title: Protocol for generating a 3D hydrogel-based tumor resection model in vitro using pancreatic cancer cells.
Article Snippet: .. MATERIALS AND EQUIPMENT REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle Medium (DMEM) Pan Biotec Cat#F0445 Fetal bovine serum Pan Biotec Cat# P40-1401 Penicillin-streptomycin Pan Biotec Cat# P06-07100 L-glutamine Pan Biotec Cat# P04-80100 Phosphate buffered saline Pan Biotec Cat# P04-36500 Hydrogel R&D Systems Cat#3432-005-01 DAPI Carl Roth Cat#6335.1 Vybrant DiD Cell Labeling Solution Thermo Fisher Cat#V22887 SYTOX Green Thermo Fisher Cat#S7020 Experimental models: Cell lines Panc-1 ATCC Cat#CRL-1469 Software and algorithms Harmony 4.9 PerkinElmer N/A BioRender BioRender RRID: SCR_018361 Other Breeding incubator Binder Cat#9010-0319 96-pin punch plate Miebach et al. 1 N/A Motorized, computer-controlled xyz table CNC Cat#13110000 Biopsy punchout KAI Europe Cat#49301 Disposable syringe BD Cat#115256 Microplate reader Tecan Cat#30050303 High-content imaging system PerkinElmer Cat#HH16000000 Complete Dulbecco’s Modified Eagle Medium (DMEM) Reagent Final concentration Amount Dulbecco’s Modified Eagle Medium (DMEM) 88% 88 mL Fetal bovine serum 10% 10 mL Penicillin-streptomycin 100 U/mL - 100 μg/mL 1 mL L-glutamine 2 mM 1 mL Total N/A 100 mL The medium can be stored for 2–3 months at 4 ◦ C. STAR Protocols 7, 104416, March 20, 2026 5 ..
Saline:Article Title: Protocol for generating a 3D hydrogel-based tumor resection model in vitro using pancreatic cancer cells.
Article Snippet: .. MATERIALS AND EQUIPMENT REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle Medium (DMEM) Pan Biotec Cat#F0445 Fetal bovine serum Pan Biotec Cat# P40-1401 Penicillin-streptomycin Pan Biotec Cat# P06-07100 L-glutamine Pan Biotec Cat# P04-80100 Phosphate buffered saline Pan Biotec Cat# P04-36500 Hydrogel R&D Systems Cat#3432-005-01 DAPI Carl Roth Cat#6335.1 Vybrant DiD Cell Labeling Solution Thermo Fisher Cat#V22887 SYTOX Green Thermo Fisher Cat#S7020 Experimental models: Cell lines Panc-1 ATCC Cat#CRL-1469 Software and algorithms Harmony 4.9 PerkinElmer N/A BioRender BioRender RRID: SCR_018361 Other Breeding incubator Binder Cat#9010-0319 96-pin punch plate Miebach et al. 1 N/A Motorized, computer-controlled xyz table CNC Cat#13110000 Biopsy punchout KAI Europe Cat#49301 Disposable syringe BD Cat#115256 Microplate reader Tecan Cat#30050303 High-content imaging system PerkinElmer Cat#HH16000000 Complete Dulbecco’s Modified Eagle Medium (DMEM) Reagent Final concentration Amount Dulbecco’s Modified Eagle Medium (DMEM) 88% 88 mL Fetal bovine serum 10% 10 mL Penicillin-streptomycin 100 U/mL - 100 μg/mL 1 mL L-glutamine 2 mM 1 mL Total N/A 100 mL The medium can be stored for 2–3 months at 4 ◦ C. STAR Protocols 7, 104416, March 20, 2026 5 ..
Labeling:Article Title: Protocol for generating a 3D hydrogel-based tumor resection model in vitro using pancreatic cancer cells.
Article Snippet: .. MATERIALS AND EQUIPMENT REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle Medium (DMEM) Pan Biotec Cat#F0445 Fetal bovine serum Pan Biotec Cat# P40-1401 Penicillin-streptomycin Pan Biotec Cat# P06-07100 L-glutamine Pan Biotec Cat# P04-80100 Phosphate buffered saline Pan Biotec Cat# P04-36500 Hydrogel R&D Systems Cat#3432-005-01 DAPI Carl Roth Cat#6335.1 Vybrant DiD Cell Labeling Solution Thermo Fisher Cat#V22887 SYTOX Green Thermo Fisher Cat#S7020 Experimental models: Cell lines Panc-1 ATCC Cat#CRL-1469 Software and algorithms Harmony 4.9 PerkinElmer N/A BioRender BioRender RRID: SCR_018361 Other Breeding incubator Binder Cat#9010-0319 96-pin punch plate Miebach et al. 1 N/A Motorized, computer-controlled xyz table CNC Cat#13110000 Biopsy punchout KAI Europe Cat#49301 Disposable syringe BD Cat#115256 Microplate reader Tecan Cat#30050303 High-content imaging system PerkinElmer Cat#HH16000000 Complete Dulbecco’s Modified Eagle Medium (DMEM) Reagent Final concentration Amount Dulbecco’s Modified Eagle Medium (DMEM) 88% 88 mL Fetal bovine serum 10% 10 mL Penicillin-streptomycin 100 U/mL - 100 μg/mL 1 mL L-glutamine 2 mM 1 mL Total N/A 100 mL The medium can be stored for 2–3 months at 4 ◦ C. STAR Protocols 7, 104416, March 20, 2026 5 ..
Software:Article Title: Protocol for generating a 3D hydrogel-based tumor resection model in vitro using pancreatic cancer cells.
Article Snippet: .. MATERIALS AND EQUIPMENT REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle Medium (DMEM) Pan Biotec Cat#F0445 Fetal bovine serum Pan Biotec Cat# P40-1401 Penicillin-streptomycin Pan Biotec Cat# P06-07100 L-glutamine Pan Biotec Cat# P04-80100 Phosphate buffered saline Pan Biotec Cat# P04-36500 Hydrogel R&D Systems Cat#3432-005-01 DAPI Carl Roth Cat#6335.1 Vybrant DiD Cell Labeling Solution Thermo Fisher Cat#V22887 SYTOX Green Thermo Fisher Cat#S7020 Experimental models: Cell lines Panc-1 ATCC Cat#CRL-1469 Software and algorithms Harmony 4.9 PerkinElmer N/A BioRender BioRender RRID: SCR_018361 Other Breeding incubator Binder Cat#9010-0319 96-pin punch plate Miebach et al. 1 N/A Motorized, computer-controlled xyz table CNC Cat#13110000 Biopsy punchout KAI Europe Cat#49301 Disposable syringe BD Cat#115256 Microplate reader Tecan Cat#30050303 High-content imaging system PerkinElmer Cat#HH16000000 Complete Dulbecco’s Modified Eagle Medium (DMEM) Reagent Final concentration Amount Dulbecco’s Modified Eagle Medium (DMEM) 88% 88 mL Fetal bovine serum 10% 10 mL Penicillin-streptomycin 100 U/mL - 100 μg/mL 1 mL L-glutamine 2 mM 1 mL Total N/A 100 mL The medium can be stored for 2–3 months at 4 ◦ C. STAR Protocols 7, 104416, March 20, 2026 5 ..
Imaging:Article Title: Protocol for generating a 3D hydrogel-based tumor resection model in vitro using pancreatic cancer cells.
Article Snippet: .. MATERIALS AND EQUIPMENT REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle Medium (DMEM) Pan Biotec Cat#F0445 Fetal bovine serum Pan Biotec Cat# P40-1401 Penicillin-streptomycin Pan Biotec Cat# P06-07100 L-glutamine Pan Biotec Cat# P04-80100 Phosphate buffered saline Pan Biotec Cat# P04-36500 Hydrogel R&D Systems Cat#3432-005-01 DAPI Carl Roth Cat#6335.1 Vybrant DiD Cell Labeling Solution Thermo Fisher Cat#V22887 SYTOX Green Thermo Fisher Cat#S7020 Experimental models: Cell lines Panc-1 ATCC Cat#CRL-1469 Software and algorithms Harmony 4.9 PerkinElmer N/A BioRender BioRender RRID: SCR_018361 Other Breeding incubator Binder Cat#9010-0319 96-pin punch plate Miebach et al. 1 N/A Motorized, computer-controlled xyz table CNC Cat#13110000 Biopsy punchout KAI Europe Cat#49301 Disposable syringe BD Cat#115256 Microplate reader Tecan Cat#30050303 High-content imaging system PerkinElmer Cat#HH16000000 Complete Dulbecco’s Modified Eagle Medium (DMEM) Reagent Final concentration Amount Dulbecco’s Modified Eagle Medium (DMEM) 88% 88 mL Fetal bovine serum 10% 10 mL Penicillin-streptomycin 100 U/mL - 100 μg/mL 1 mL L-glutamine 2 mM 1 mL Total N/A 100 mL The medium can be stored for 2–3 months at 4 ◦ C. STAR Protocols 7, 104416, March 20, 2026 5 ..
Concentration Assay:Article Title: Protocol for generating a 3D hydrogel-based tumor resection model in vitro using pancreatic cancer cells.
Article Snippet: .. MATERIALS AND EQUIPMENT REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle Medium (DMEM) Pan Biotec Cat#F0445 Fetal bovine serum Pan Biotec Cat# P40-1401 Penicillin-streptomycin Pan Biotec Cat# P06-07100 L-glutamine Pan Biotec Cat# P04-80100 Phosphate buffered saline Pan Biotec Cat# P04-36500 Hydrogel R&D Systems Cat#3432-005-01 DAPI Carl Roth Cat#6335.1 Vybrant DiD Cell Labeling Solution Thermo Fisher Cat#V22887 SYTOX Green Thermo Fisher Cat#S7020 Experimental models: Cell lines Panc-1 ATCC Cat#CRL-1469 Software and algorithms Harmony 4.9 PerkinElmer N/A BioRender BioRender RRID: SCR_018361 Other Breeding incubator Binder Cat#9010-0319 96-pin punch plate Miebach et al. 1 N/A Motorized, computer-controlled xyz table CNC Cat#13110000 Biopsy punchout KAI Europe Cat#49301 Disposable syringe BD Cat#115256 Microplate reader Tecan Cat#30050303 High-content imaging system PerkinElmer Cat#HH16000000 Complete Dulbecco’s Modified Eagle Medium (DMEM) Reagent Final concentration Amount Dulbecco’s Modified Eagle Medium (DMEM) 88% 88 mL Fetal bovine serum 10% 10 mL Penicillin-streptomycin 100 U/mL - 100 μg/mL 1 mL L-glutamine 2 mM 1 mL Total N/A 100 mL The medium can be stored for 2–3 months at 4 ◦ C. STAR Protocols 7, 104416, March 20, 2026 5 ..
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